› 5es8

X-ray diffraction
2.55Å resolution

Crystal structure of the initiation module of LgrA in the thiolation state

Released:
DOI: 10.2210/pdb5es8/pdb
Model geometry
Fit model/data
PDB entry 5es8 coloured by chain, front view.
PDB entry 5es8 coloured by chain, side view.
PDB entry 5es8 coloured by chain, top view.
Source organism: Brevibacillus parabrevis
Primary publication:
Synthetic cycle of the initiation module of a formylating nonribosomal peptide synthetase.
Reimer JM, Aloise MN, Harrison PM, Schmeing TM
Nature 529 239-42 (2016)
PMID: 26762462

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Linear gramicidin synthase subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 776 amino acids
Theoretical weight: 88.06 KDa
Source organism: Brevibacillus parabrevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70LM7 (Residues: 2-766; Coverage: 34%)
Gene name: lgrA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 5S4 2 x 5S4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 76.55Å b: 101.05Å c: 138.74Å
α: 90° β: 91.77° γ: 90°
R-values:
R R work R free
0.227 0.225 0.274
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Nonribosomal Peptide Synthesis-Principles and Prospects.
Süssmuth et al. (2017)
18 more

7 mentions without citation

Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study.
Ronnebaum et al. (2018)
6 more