5esp

X-ray diffraction
3Å resolution

Crystal Structure of LAGLIDADG Meganuclease I-PanMI with coordinated Calcium ions

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-118643 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Uncharacterized 49.1 kDa protein in ND3 intron Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 34 KDa
Source organism: Podospora anserina S mat+
Expression system: Escherichia coli
UniProt:
  • Canonical: P15563 (Residues: 136-433; Coverage: 69%)
Sequence domains: LAGLIDADG endonuclease
DNA (27-MER) Chains: C, G
Molecule details ›
Chains: C, G
Length: 27 nucleotides
Theoretical weight: 8.09 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (27-MER) Chains: D, H
Molecule details ›
Chains: D, H
Length: 27 nucleotides
Theoretical weight: 8.5 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 69.555Å b: 78.442Å c: 101.023Å
α: 90° β: 92.38° γ: 90°
R-values:
R R work R free
0.23 0.226 0.269
Expression systems:
  • Escherichia coli
  • Not provided