› 5esr

X-ray diffraction
1.48Å resolution

Crystal structure of haloalkane dehalogenase (DccA) from Caulobacter crescentus

Released:
DOI: 10.2210/pdb5esr/pdb
PDB entry 5esr coloured by chain, front view.
PDB entry 5esr coloured by chain, side view.
PDB entry 5esr coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Primary publication:
Biochemical characterization of two haloalkane dehalogenases: DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea.
Carlucci L, Zhou E, Malashkevich VN, Almo SC, Mundorff EC
Protein Sci 25 877-86 (2016)
PMID: 26833751

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34.02 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9A919 (Residues: 1-302; Coverage: 100%)
Gene names: CC_1175, dhmA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CO 6 x CO
Ligand MG 2 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 79.377Å b: 79.643Å c: 94.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.127 0.126 0.149
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Microbial Synthesis and Transformation of Inorganic and Organic Chlorine Compounds.
Atashgahi et al. (2018)
5 other articles

2 mentions without citation

Biochemical characterization of two haloalkane dehalogenases: DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea.
Carlucci et al. (2016)
1 more