› 5ew4

X-ray diffraction
1.47Å resolution

Crystal structure of C9ORF72 Antisense CCCCGG repeat RNA associated with Lou Gehrig's disease and frontotemporal dementia, crystallized with Sr2+

Released:
DOI: 10.2210/pdb5ew4/pdb
PDB entry 5ew4 coloured by chain, front view.
PDB entry 5ew4 coloured by chain, side view.
PDB entry 5ew4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pathogenic C9ORF72 Antisense Repeat RNA Forms a Double Helix with Tandem C:C Mismatches.
Dodd DW, Tomchick DR, Corey DR, Gagnon KT
Biochemistry 55 1283-6 (2016)
PMID: 26878348

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196926 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*CP*CP*CP*CP*GP*GP*CP*CP*CP*CP*GP*GP*CP*CP*CP*CP*GP*GP*CP*CP*CP*C)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 22 nucleotides
Theoretical weight: 6.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand K 9 x K
Ligand SR 1 x SR
Ligand MG 2 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 41.02Å b: 47.615Å c: 58.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.239
Expression system: Not provided

Quick links

Citations

12 review citations

RAN translation-What makes it run?
Green et al. (2016)
11 more

2 mentions without citation

Mg2+ ions: do they bind to nucleobase nitrogens?
Leonarski et al. (2017)
1 more