› 5ex7

X-ray diffraction
2.6Å resolution

Crystal structure of Brat NHL domain in complex with an 8-nt hunchback mRNA

Released:
DOI: 10.2210/pdb5ex7/pdb
PDB entry 5ex7 coloured by chain, front view.
PDB entry 5ex7 coloured by chain, side view.
PDB entry 5ex7 coloured by chain, top view.
Source organisms:
Entry authors: Wang Y, Yu Z, Wang M, Liu CP, Yang N, Xu RM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185326 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Brain tumor protein Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 32.36 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8MQJ9 (Residues: 758-1037; Coverage: 27%)
Gene names: CG10719, brat
Sequence domains: NHL repeat
Structure domains: TolB, C-terminal domain
RNA (5'-R(P*UP*UP*UP*GP*UP*UP*GP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.48 KDa
Source organism: Drosophila
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 150.518Å b: 150.518Å c: 37.921Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.181 0.231
Expression systems:
  • Escherichia coli
  • Not provided

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