› 5ey0

X-ray diffraction
1.6Å resolution

Crystal structure of CodY from Staphylococcus aureus with GTP and Ile

Released:
DOI: 10.2210/pdb5ey0/pdb
Model geometry
Fit model/data
PDB entry 5ey0 coloured by chain, front view.
PDB entry 5ey0 coloured by chain, side view.
PDB entry 5ey0 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu3
Primary publication:
The structure of the pleiotropic transcription regulator CodY provides insight into its GTP-sensing mechanism.
Han AR, Kang HR, Son J, Kwon DH, Kim S, Lee WC, Song HK, Song MJ, Hwang KY
OpenAccess logo Nucleic Acids Res 44 9483-9493 (2016)
PMID: 27596595

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Global transcriptional regulator CodY Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 30.74 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu3
Expression system: Escherichia coli
UniProt:
  • Canonical: A7X1N2 (Residues: 1-257; Coverage: 100%)
Gene names: SAHV_1245, codY
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ILE 2 x ILE
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 45.894Å b: 76.028Å c: 158.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.209
Expression system: Escherichia coli

Quick links

Citations

1 review citation

CodY, a master integrator of metabolism and virulence in Gram-positive bacteria.
Brinsmade SR. (2017)
10 other articles

3 mentions without citation

Repression of branched-chain amino acid synthesis in Staphylococcus aureus is mediated by isoleucine via CodY, and by a leucine-rich attenuator peptide.
Kaiser et al. (2018)
2 more