› 5eyo

X-ray diffraction
2.39Å resolution

The crystal structure of the Max bHLH domain in complex with 5-carboxyl cytosine DNA

Released:
DOI: 10.2210/pdb5eyo/pdb
PDB entry 5eyo coloured by chain, front view.
PDB entry 5eyo coloured by chain, side view.
PDB entry 5eyo coloured by chain, top view.
Source organisms:
Primary publication:
MAX is an epigenetic sensor of 5-carboxylcytosine and is altered in multiple myeloma.
Wang D, Hashimoto H, Zhang X, Barwick BG, Lonial S, Boise LH, Vertino PM, Cheng X
Nucleic Acids Res 45 2396-2407 (2017)
PMID: 27903915

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118654 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein max Chains: A, C
Molecule details ›
Chains: A, C
Length: 88 amino acids
Theoretical weight: 10.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61244 (Residues: 22-107; Coverage: 54%)
Gene names: BHLHD4, MAX
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(*AP*GP*TP*AP*GP*CP*AP*(1CC)P*GP*TP*GP*CP*TP*AP*CP*T)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 1CC 2 x 1CC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 32.75Å b: 306.16Å c: 49.06Å
α: 90° β: 103.79° γ: 90°
R-values:
R R work R free
0.204 0.203 0.229
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

TET-mediated active DNA demethylation: mechanism, function and beyond.
Wu et al. (2017)
12 more

5 mentions without citation

Low-Affinity Binding Sites and the Transcription Factor Specificity Paradox in Eukaryotes.
Kribelbauer et al. (2019)
4 more