› 5eyt

X-ray diffraction
2.36Å resolution

Crystal Structure of Adenylosuccinate Lyase from Schistosoma mansoni in complex with AMP

Released:
DOI: 10.2210/pdb5eyt/pdb
PDB entry 5eyt coloured by chain, front view.
PDB entry 5eyt coloured by chain, side view.
PDB entry 5eyt coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Structural and kinetic analysis of Schistosoma mansoni Adenylosuccinate Lyase (SmADSL).
Romanello L, Serrão VHB, Torini JR, Bird LE, Nettleship JE, Rada H, Reddivari Y, Owens RJ, DeMarco R, Brandão-Neto J, Pereira HD
Mol Biochem Parasitol 214 27-35 (2017)
PMID: 28347672

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chain: A
Molecule details ›
Chain: A
Length: 497 amino acids
Theoretical weight: 56.57 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli
UniProt:
  • Canonical: G4VQX9 (Residues: 1-480; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I222
Unit cell:
a: 70.466Å b: 73.114Å c: 180.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.231
Expression system: Escherichia coli

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Citations

1 review citation

Pyrimidine metabolism in schistosomes: A comparison with other parasites and the search for potential chemotherapeutic targets.
El Kouni MH. (2017)
6 other articles