› 5ez2

X-ray diffraction
1.85Å resolution

Sandercyanin Fluorescent Protein (SFP)

Released:
DOI: 10.2210/pdb5ez2/pdb
PDB entry 5ez2 coloured by chain, front view.
PDB entry 5ez2 coloured by chain, side view.
PDB entry 5ez2 coloured by chain, top view.
Source organism: Sander vitreus
Primary publication:
Blue protein with red fluorescence.
Ghosh S, Yu CL, Ferraro DJ, Sudha S, Pal SK, Schaefer WF, Gibson DT, Ramaswamy S
Proc Natl Acad Sci U S A 113 11513-11518 (2016)
PMID: 27688756

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipocalin/cytosolic fatty-acid binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.16 KDa
Source organism: Sander vitreus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1D5B367 (Residues: 1-183; Coverage: 100%)
Sequence domains: Lipocalin-like domain
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand BLA 2 x BLA
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6322
Unit cell:
a: 159.266Å b: 159.266Å c: 84.157Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Sea as a color palette: the ecology and evolution of fluorescence.
Macel et al. (2020)
8 other articles

2 mentions without citation

Blue protein with red fluorescence.
Ghosh et al. (2016)
1 more