› 5ezb

X-ray diffraction
2.3Å resolution

Chicken prestin STAS domain

Released:
DOI: 10.2210/pdb5ezb/pdb
PDB entry 5ezb coloured by chain, front view.
PDB entry 5ezb coloured by chain, side view.
PDB entry 5ezb coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
The STAS domain of mammalian SLC26A5 prestin harbours an anion-binding site.
Lolli G, Pasqualetto E, Costanzi E, Bonetto G, Battistutta R
Biochem J 473 365-70 (2016)
PMID: 26635354

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prestin Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 15.98 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0FKN5 (Residues: 511-569, 652-733; Coverage: 19%)
Gene name: SLC26A5
Sequence domains: STAS domain
Structure domains: STAS domain

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
Ligand NA 1 x NA
Ligand OXL 1 x OXL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Unit cell:
a: 81.551Å b: 56.919Å c: 101.14Å
α: 90° β: 96.19° γ: 90°
R-values:
R R work R free
0.222 0.22 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Chansporter complexes in cell signaling.
Abbott GW. (2017)
1 more

1 mention without citation

Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport.
Walter et al. (2019)