› 5f0b

X-ray diffraction
2.15Å resolution

Crystal structure of murine neuroglobin mutant F106W at 280 MPa pressure

Released:
DOI: 10.2210/pdb5f0b/pdb
PDB entry 5f0b coloured by chain, front view.
PDB entry 5f0b coloured by chain, side view.
PDB entry 5f0b coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Determinants of neuroglobin plasticity highlighted by joint coarse-grained simulations and high pressure crystallography.
Colloc'h N, Sacquin-Mora S, Avella G, Dhaussy AC, Prangé T, Vallone B, Girard E
Sci Rep 7 1858 (2017)
PMID: 28500341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuroglobin Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ER97 (Residues: 3-151; Coverage: 99%)
Gene name: Ngb
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID09
Spacegroup: R32
Unit cell:
a: 86.212Å b: 86.212Å c: 114.459Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.171 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Mapping Hydrophobic Tunnels and Cavities in Neuroglobin with Noble Gas under Pressure.
Colloc'h et al. (2017)
2 more

1 mention without citation

Determinants of neuroglobin plasticity highlighted by joint coarse-grained simulations and high pressure crystallography.
Colloc'h et al. (2017)