› 5f15

X-ray diffraction
3.2Å resolution

Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate

Released:
DOI: 10.2210/pdb5f15/pdb
PDB entry 5f15 coloured by chain, front view.
PDB entry 5f15 coloured by chain, side view.
PDB entry 5f15 coloured by chain, top view.
Source organism: Cupriavidus metallidurans CH34
Primary publication:
Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation.
Petrou VI, Herrera CM, Schultz KM, Clarke OB, Vendome J, Tomasek D, Banerjee S, Rajashankar KR, Belcher Dufrisne M, Kloss B, Kloppmann E, Rost B, Klug CS, Trent MS, Shapiro L, Mancia F
Science 351 608-12 (2016)
PMID: 26912703

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferases of PMT family Chain: A
Molecule details ›
Chain: A
Length: 578 amino acids
Theoretical weight: 63.21 KDa
Source organism: Cupriavidus metallidurans CH34
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1LDT6 (Residues: 1-575; Coverage: 100%)
Gene name: Rmet_4828
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand MPG 76 x MPG
Ligand EPE 1 x EPE
Ligand PO4 1 x PO4
Ligand CL 2 x CL
Ligand 5TR 1 x 5TR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 59.648Å b: 80.322Å c: 150.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Regulating polymyxin resistance in Gram-negative bacteria: roles of two-component systems PhoPQ and PmrAB.
Huang et al. (2020)
10 more

12 mentions without citation

Structural basis for catalysis at the membrane-water interface.
Dufrisne et al. (2017)
11 more