› 5f1r

X-ray diffraction
2.25Å resolution

The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with a ring-fused 2-pyridone (C10)

Released:
DOI: 10.2210/pdb5f1r/pdb
PDB entry 5f1r coloured by chain, front view.
PDB entry 5f1r coloured by chain, side view.
PDB entry 5f1r coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Attenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA.
Good JA, Andersson C, Hansen S, Wall J, Krishnan KS, Begum A, Grundström C, Niemiec MS, Vaitkevicius K, Chorell E, Wittung-Stafshede P, Sauer UH, Sauer-Eriksson AE, Almqvist F, Johansson J
Cell Chem Biol 23 404-14 (2016)
PMID: 26991105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.33 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 42O 2 x 42O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 56.34Å b: 80.97Å c: 62.34Å
α: 90° β: 112.54° γ: 90°
R-values:
R R work R free
0.203 0.198 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Role of Stress and Stress Adaptations in Determining the Fate of the Bacterial Pathogen Listeria monocytogenes in the Food Chain.
NicAogáin et al. (2016)
1 more

4 mentions without citation

Attenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA.
Good et al. (2016)
3 more