› 5f23

X-ray diffraction
1.5Å resolution

Crystal structure of NH(3)-dependent NAD(+) synthetase Pseudomonas aeruginosa in complex with NAD

Released:
DOI: 10.2210/pdb5f23/pdb
Model geometry
Fit model/data
PDB entry 5f23 coloured by chain, front view.
PDB entry 5f23 coloured by chain, side view.
PDB entry 5f23 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 30.76 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HUP3 (Residues: 1-275; Coverage: 100%)
Gene names: PA4920, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand NAD 1 x NAD
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 111.87Å b: 41.19Å c: 64.13Å
α: 90° β: 113.54° γ: 90°
R-values:
R R work R free
0.14 0.138 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links