› 5f3h

X-ray diffraction
2.7Å resolution

Structure of myostatin in complex with humanized RK35 antibody

Released:
DOI: 10.2210/pdb5f3h/pdb
PDB entry 5f3h coloured by chain, front view.
PDB entry 5f3h coloured by chain, side view.
PDB entry 5f3h coloured by chain, top view.
Source organisms:
Primary publication:
Beyond CDR-grafting: Structure-guided humanization of framework and CDR regions of an anti-myostatin antibody.
Apgar JR, Mader M, Agostinelli R, Benard S, Bialek P, Johnson M, Gao Y, Krebs M, Owens J, Parris K, St Andre M, Svenson K, Morris C, Tchistiakova L
MAbs 8 1302-1318 (2016)
PMID: 27625211

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229745 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
humanized RK35 antibody heavy chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 221 amino acids
Theoretical weight: 23.37 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
humanized RK35 antibody light chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 214 amino acids
Theoretical weight: 23.37 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Growth/differentiation factor 8 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 108 amino acids
Theoretical weight: 12.31 KDa
Source organism: Homo sapiens
Expression system: Cricetus
UniProt:
  • Canonical: O14793 (Residues: 268-375; Coverage: 31%)
Gene names: GDF8, MSTN
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 75.138Å b: 80.916Å c: 101.401Å
α: 88.33° β: 103.78° γ: 92.4°
R-values:
R R work R free
0.221 0.218 0.27
Expression systems:
  • Cricetulus griseus
  • Cricetus

Quick links

Citations

5 review citations

Progress and Challenges in the Design and Clinical Development of Antibodies for Cancer Therapy.
Almagro et al. (2017)
4 more

3 mentions without citation

Beyond CDR-grafting: Structure-guided humanization of framework and CDR regions of an anti-myostatin antibody.
Apgar et al. (2016)
2 more