› 5f4h

X-ray diffraction
2.7Å resolution

Archael RuvB-like Holiday junction helicase

Released:
DOI: 10.2210/pdb5f4h/pdb
PDB entry 5f4h coloured by chain, front view.
PDB entry 5f4h coloured by chain, side view.
PDB entry 5f4h coloured by chain, top view.
Source organism: Sulfolobus islandicus L.S.2.15
Primary publication:
Structure and Function of a Novel ATPase that Interacts with Holliday Junction Resolvase Hjc and Promotes Branch Migration.
Zhai B, DuPrez K, Doukov TI, Li H, Huang M, Shang G, Ni J, Gu L, Shen Y, Fan L
J Mol Biol 429 1009-1029 (2017)
PMID: 28238763

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction branch migration ATPase PINA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 505 amino acids
Theoretical weight: 57.24 KDa
Source organism: Sulfolobus islandicus L.S.2.15
Expression system: Escherichia coli
UniProt:
  • Canonical: F0NID4 (Residues: 1-505; Coverage: 100%)
Gene names: SiRe_1432, pina
Sequence domains: Type II/IV secretion system protein

Ligands and Environments

2 bound ligands:
Ligand GOL 21 x GOL
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 101.72Å b: 148.98Å c: 122.13Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.229 0.227 0.265
Expression system: Escherichia coli

Quick links

Citations

1 review citation

DNA repair in the archaea-an emerging picture.
White et al. (2018)
3 other articles

2 mentions without citation

Structure and Function of a Novel ATPase that Interacts with Holliday Junction Resolvase Hjc and Promotes Branch Migration.
Zhai et al. (2017)
1 more