› 5f4q

X-ray diffraction
1.8Å resolution

Crystal structure of the human egg surface protein Juno

Released:
DOI: 10.2210/pdb5f4q/pdb
PDB entry 5f4q coloured by chain, front view.
PDB entry 5f4q coloured by chain, side view.
PDB entry 5f4q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex.
Aydin H, Sultana A, Li S, Thavalingam A, Lee JE
Nature 534 562-5 (2016)
PMID: 27309818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sperm-egg fusion protein Juno Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 24.58 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A6ND01 (Residues: 20-228; Coverage: 91%)
Gene names: FOLR4, IZUMO1R, JUNO
Sequence domains: Folate receptor family

Ligands and Environments

3 bound ligands:
Ligand NAG 4 x NAG
Ligand CL 4 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P41
Unit cell:
a: 73.15Å b: 73.15Å c: 163.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.227
Expression system: Drosophila melanogaster

Quick links

Citations

21 review citations

The hallmarks of cell-cell fusion.
Hernández et al. (2017)
20 more

4 mentions without citation

The cell biology of fertilization: Gamete attachment and fusion.
Siu et al. (2021)
3 more