› 5f4y

X-ray diffraction
3.29Å resolution

Structure of the SD2 domain of Human Shroom2

Released:
DOI: 10.2210/pdb5f4y/pdb
PDB entry 5f4y coloured by chain, front view.
PDB entry 5f4y coloured by chain, side view.
PDB entry 5f4y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Shroom-Rho Kinase Complex Reveals a Binding Interface with Monomeric Shroom That Regulates Cell Morphology and Stimulates Kinase Activity.
Zalewski JK, Mo JH, Heber S, Heroux A, Gardner RG, Hildebrand JD, VanDemark AP
J Biol Chem 291 25364-25374 (2016)
PMID: 27758857

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein Shroom2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13796 (Residues: 1427-1610; Coverage: 11%)
Gene names: APXL, SHROOM2
Sequence domains: Apx/Shroom domain ASD2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 90.887Å b: 110Å c: 118.862Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.247 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Quantitative insights in tissue growth and morphogenesis with optogenetics.
Mim et al. (2023)
11 other articles

1 mention without citation

Structure of the Shroom-Rho Kinase Complex Reveals a Binding Interface with Monomeric Shroom That Regulates Cell Morphology and Stimulates Kinase Activity.
Zalewski et al. (2016)