› 5f4z

X-ray diffraction
1.82Å resolution

The crystal structure of an epoxide hydrolase from Streptomyces carzinostaticus subsp. neocarzinostaticus

Released:
DOI: 10.2210/pdb5f4z/pdb
PDB entry 5f4z coloured by chain, front view.
PDB entry 5f4z coloured by chain, side view.
PDB entry 5f4z coloured by chain, top view.
Source organism: Streptomyces carzinostaticus subsp. neocarzinostaticus
Entry authors: Tan K, Li H, Jedrzejczak R, BABNIGG G, BINGMAN CA, YENNAMALLI R, LOHMAN J, Chang CY, Shen B, Phillips Jr GN, Joachimiak A, Midwest Center for Structural Genomics (MCSG), Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epoxide hydrolase N-terminal domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 393 amino acids
Theoretical weight: 43.78 KDa
Source organism: Streptomyces carzinostaticus subsp. neocarzinostaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84HB1 (Residues: 1-387; Coverage: 100%)
Sequence domains: Epoxide hydrolase N terminus
Structure domains: alpha/beta hydrolase

Ligands and Environments

6 bound ligands:
Ligand GOL 7 x GOL
Ligand ACT 2 x ACT
Ligand PEG 1 x PEG
Ligand 5V4 5 x 5V4
Ligand PG4 1 x PG4
Ligand TRS 1 x TRS
1 modified residue:
Ligand MSE 35 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 179.356Å b: 93.068Å c: 134.059Å
α: 90° β: 115.33° γ: 90°
R-values:
R R work R free
0.162 0.16 0.2
Expression system: Escherichia coli BL21(DE3)

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