› 5f5l

X-ray diffraction
1.68Å resolution

The structure of monooxygenase KstA11 in the biosynthetic pathway of kosinostatin

Released:
DOI: 10.2210/pdb5f5l/pdb
PDB entry 5f5l coloured by chain, front view.
PDB entry 5f5l coloured by chain, side view.
PDB entry 5f5l coloured by chain, top view.
Source organism: Micromonospora sp. TP-A0468
Primary publication:
Hydroxyl regioisomerization of anthracycline catalyzed by a four-enzyme cascade.
Zhang Z, Gong YK, Zhou Q, Hu Y, Ma HM, Chen YS, Igarashi Y, Pan L, Tang GL
Proc Natl Acad Sci U S A 114 1554-1559 (2017)
PMID: 28137838

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NmrA-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 31.02 KDa
Source organism: Micromonospora sp. TP-A0468
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A023GUL3 (Residues: 4-293; Coverage: 97%)
Sequence domains: NmrA-like family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand DTT 1 x DTT
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P4212
Unit cell:
a: 93.748Å b: 93.748Å c: 71.182Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.129 0.177
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

Development of a drug discovery approach from microbes with a special focus on isolation sources and taxonomy.
Igarashi Y. (2023)
2 other articles

1 mention without citation

Hydroxyl regioisomerization of anthracycline catalyzed by a four-enzyme cascade.
Zhang et al. (2017)