› 5f72

X-ray diffraction
1.85Å resolution

De novo design and crystallographic validation of antibodies targeting a pre-selected epitope

Released:
DOI: 10.2210/pdb5f72/pdb
PDB entry 5f72 coloured by chain, front view.
PDB entry 5f72 coloured by chain, side view.
PDB entry 5f72 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Liu X, Taylor RD, Griffin L, Coker S, Adams R, Ceska T, Shi J, Lawson ADG, Baker T

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212019 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kelch-like ECH-associated protein 1 Chains: C, K
Molecule details ›
Chains: C, K
Length: 296 amino acids
Theoretical weight: 32.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14145 (Residues: 321-611; Coverage: 47%)
Gene names: INRF2, KEAP1, KIAA0132, KLHL19
Sequence domains: Kelch motif
Structure domains: Kelch-type beta propeller
Single chain Fv from a Fab Chains: S, T
Molecule details ›
Chains: S, T
Length: 257 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 70.499Å b: 69.808Å c: 99.562Å
α: 90° β: 92.23° γ: 90°
R-values:
R R work R free
0.225 0.223 0.265
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

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