› 5f8h

X-ray diffraction
2.45Å resolution

Enterovirus 71 Polymerase Elongation Complex (C1S1/2 Form)

Released:
DOI: 10.2210/pdb5f8h/pdb
PDB entry 5f8h coloured by chain, front view.
PDB entry 5f8h coloured by chain, side view.
PDB entry 5f8h coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation.
Shu B, Gong P
Proc Natl Acad Sci U S A 113 E4005-14 (2016)
PMID: 27339134

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122996 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Genome polyprotein Chain: A
Molecule details ›
Chain: A
Length: 468 amino acids
Theoretical weight: 53.38 KDa
Source organism: Enterovirus A71
Expression system: Escherichia coli
UniProt:
  • Canonical: E5RPG2 (Residues: 1732-2193; Coverage: 21%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
RNA (35-MER) Chain: B
Molecule details ›
Chain: B
Length: 35 nucleotides
Theoretical weight: 11.26 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*UP*GP*UP*UP*CP*GP*AP*CP*GP*AP*GP*AP*GP*AP*GP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 5.2 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand ZN 1 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 63.344Å b: 77.081Å c: 149.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.244
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

28 review citations

Recent Developments on Therapeutic and Diagnostic Approaches for COVID-19.
Majumder et al. (2021)
27 more

2 mentions without citation

Visualizing the Nucleotide Addition Cycle of Viral RNA-Dependent RNA Polymerase.
Wu et al. (2018)
1 more