› 5f90

X-ray diffraction
1.64Å resolution

Crystal structure of a Crenomytilus grayanus lectin in complex with Gb3 allyl

Released:
DOI: 10.2210/pdb5f90/pdb
PDB entry 5f90 coloured by chain, front view.
PDB entry 5f90 coloured by chain, side view.
PDB entry 5f90 coloured by chain, top view.
Source organism: Crenomytilus grayanus
Primary publication:
A Multivalent Marine Lectin from Crenomytilus grayanus Possesses Anti-cancer Activity through Recognizing Globotriose Gb3.
Liao JH, Chien CT, Wu HY, Huang KF, Wang I, Ho MR, Tu IF, Lee IM, Li W, Shih YL, Wu CY, Lukyanov PA, Hsu ST, Wu SH
J Am Chem Soc 138 4787-95 (2016)
PMID: 27010847

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Galactose-binding lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 17.88 KDa
Source organism: Crenomytilus grayanus
Expression system: Escherichia coli
UniProt:
  • Canonical: H2FH31 (Residues: 1-150; Coverage: 100%)
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, GLA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, GLA
Carbohydrate polymer
2 bound ligands:
Ligand LMR 1 x LMR
Ligand 5VQ 3 x 5VQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 53.043Å b: 72.464Å c: 94.361Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.117 0.167
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Antiviral lectins: Selective inhibitors of viral entry.
Mitchell et al. (2017)
7 more

4 mentions without citation

Structural Diversities of Lectins Binding to the Glycosphingolipid Gb3.
Siukstaite et al. (2021)
3 more