› 5f9p

X-ray diffraction
2.08Å resolution

Crystal structure study of anthrone oxidase-like protein

Released:
DOI: 10.2210/pdb5f9p/pdb
PDB entry 5f9p coloured by chain, front view.
PDB entry 5f9p coloured by chain, side view.
PDB entry 5f9p coloured by chain, top view.
Source organism: Streptomyces ambofaciens
Primary publication:
Structure and Function of a C-C Bond Cleaving Oxygenase in Atypical Angucycline Biosynthesis.
Pan G, Gao X, Fan K, Liu J, Meng B, Gao J, Wang B, Zhang C, Han H, Ai G, Chen Y, Wu D, Liu ZJ, Yang K
ACS Chem Biol 12 142-152 (2017)
PMID: 28103689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABM domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 26.75 KDa
Source organism: Streptomyces ambofaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6VMI5 (Residues: 2-232; Coverage: 100%)
Gene names: DSMT0187, alpJ
Sequence domains: Antibiotic biosynthesis monooxygenase

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P62
Unit cell:
a: 90.273Å b: 90.273Å c: 94.745Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.155 0.206
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

New insights into bacterial type II polyketide biosynthesis.
Zhang et al. (2017)
1 more