› 5fa9

X-ray diffraction
2.3Å resolution

Bifunctional Methionine Sulfoxide Reductase AB (MsrAB) from Treponema denticola

Released:
DOI: 10.2210/pdb5fa9/pdb
Model geometry
Fit model/data
PDB entry 5fa9 coloured by chain, front view.
PDB entry 5fa9 coloured by chain, side view.
PDB entry 5fa9 coloured by chain, top view.
Source organism: Treponema denticola ATCC 35405
Primary publication:
Essential Role of the Linker Region in the Higher Catalytic Efficiency of a Bifunctional MsrA-MsrB Fusion Protein.
Han AR, Kim MJ, Kwak GH, Son J, Hwang KY, Kim HY
Biochemistry 55 5117-27 (2016)
PMID: 27551953

Function and Biology Details

Reactions catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (R)-S-oxide + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide methionine sulfoxide reductase MsrA Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 36.91 KDa
Source organism: Treponema denticola ATCC 35405
Expression system: Escherichia coli
UniProt:
  • Canonical: Q73PT7 (Residues: 1-317; Coverage: 100%)
Gene names: TDE_0709, msrA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand D1D 2 x D1D
Ligand DTT 5 x DTT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 144.956Å b: 95.584Å c: 81.39Å
α: 90° β: 116.24° γ: 90°
R-values:
R R work R free
0.206 0.205 0.234
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Reactive species and pathogen antioxidant networks during phagocytosis.
Piacenza et al. (2019)
2 more

1 mention without citation

Structural Insights into a Bifunctional Peptide Methionine Sulfoxide Reductase MsrA/B Fusion Protein from Helicobacter pylori.
Kim et al. (2021)