› 5fai

X-ray diffraction
1.8Å resolution

EMG1 N1-Specific Pseudouridine Methyltransferase

Released:
DOI: 10.2210/pdb5fai/pdb
PDB entry 5fai coloured by chain, front view.
PDB entry 5fai coloured by chain, side view.
PDB entry 5fai coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: DONG A, ZENG H, LI Y, TEMPEL W, Bountra C, Arrowsmith CH, Edwards AM, BROWN PJ, WU H, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase NEP1 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92979 (Residues: 14-244; Coverage: 95%)
Gene names: C2F, EMG1
Sequence domains: EMG1/NEP1 methyltransferase
Structure domains: Alpha/beta knot

Ligands and Environments

3 bound ligands:
Ligand SAH 1 x SAH
Ligand CIT 2 x CIT
Ligand UNX 12 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P6522
Unit cell:
a: 86.118Å b: 86.118Å c: 125.178Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.168 0.183
Expression system: Escherichia coli BL21(DE3)

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