› 5fdf

X-ray diffraction
1.76Å resolution

Crystal structure of the monoclinic form of Thermotoga maritima Acetyl Esterase TM0077 (apo structure) at 1.76 Angstrom resolution

Released:
DOI: 10.2210/pdb5fdf/pdb
PDB entry 5fdf coloured by chain, front view.
PDB entry 5fdf coloured by chain, side view.
PDB entry 5fdf coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
An extended loop in CE7 carbohydrate esterase family is dispensable for oligomerization but required for activity and thermostability.
Singh MK, Manoj N
J Struct Biol 194 434-45 (2016)
PMID: 27085421

Function and Biology Details

Reactions catalysed: 
Cephalosporin C + H(2)O = deacetylcephalosporin C + acetate
Deacetylation of xylans and xylo-oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cephalosporin-C deacetylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 337 amino acids
Theoretical weight: 38.66 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WXT2 (Residues: 1-325; Coverage: 100%)
Gene names: TM_0077, axeA
Sequence domains: Acetyl xylan esterase (AXE1)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 89.76Å b: 116.2Å c: 103.29Å
α: 90° β: 109.97° γ: 90°
R-values:
R R work R free
0.162 0.16 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Properties and biotechnological applications of microbial deacetylase.
Zou et al. (2023)
3 other articles

2 mentions without citation

Crystal structure and functional characterization of a cold-active acetyl xylan esterase (PbAcE) from psychrophilic soil microbe Paenibacillus sp.
Park et al. (2018)
1 more