› 5fdn

X-ray diffraction
2.2Å resolution

Crystal structure of phosphoenolpyruvate carboxylase from Arabidopsis thaliana in complex with aspartate and citrate

Released:
DOI: 10.2210/pdb5fdn/pdb
PDB entry 5fdn coloured by chain, front view.
PDB entry 5fdn coloured by chain, side view.
PDB entry 5fdn coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Connell MB, Lee MJY, Plaxton WC, Jia Z

Function and Biology Details

Reaction catalysed: 
Phosphate + oxaloacetate = H(2)O + phosphoenolpyruvate + HCO(3)(-)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxylase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 987 amino acids
Theoretical weight: 112.34 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q84VW9 (Residues: 1-968; Coverage: 100%)
Gene names: At3g14940, K15M2.8, PEPC, PPC, PPC3
Sequence domains: Phosphoenolpyruvate carboxylase

Ligands and Environments

2 bound ligands:
Ligand ASP 2 x ASP
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C222
Unit cell:
a: 264.26Å b: 268.13Å c: 77.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.192
Expression system: Escherichia coli BL21

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