› 5fgb

X-ray diffraction
1.65Å resolution

Three dimensional structure of broadly neutralizing human anti - Hepatitis C virus (HCV) glycoprotein E2 Fab fragment HC33.4

Released:
DOI: 10.2210/pdb5fgb/pdb
PDB entry 5fgb coloured by chain, front view.
PDB entry 5fgb coloured by chain, side view.
PDB entry 5fgb coloured by chain, top view.
Source organisms:
Primary publication:
Antibody Response to Hypervariable Region 1 Interferes with Broadly Neutralizing Antibodies to Hepatitis C Virus.
Keck ZY, Girard-Blanc C, Wang W, Lau P, Zuiani A, Rey FA, Krey T, Diamond MS, Foung SK
J Virol 90 3112-22 (2016)
PMID: 26739044

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210948 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Anti-HCV E2 Fab HC84-1 light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Drosophila
Structure domains: Immunoglobulins
Anti-HCV E2 Fab HC84-1 heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 269 amino acids
Theoretical weight: 28.39 KDa
Source organism: Homo sapiens
Expression system: Drosophila
Structure domains: Immunoglobulins
Envelope glycoprotein E2 Chains: F, G
Molecule details ›
Chains: F, G
Length: 21 amino acids
Theoretical weight: 2.3 KDa
Source organism: Hepatitis C virus (isolate H)
Expression system: Not provided
UniProt:
  • Canonical: P27958 (Residues: 405-425; Coverage: 1%)

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 49.81Å b: 149.43Å c: 67.95Å
α: 90° β: 90.08° γ: 90°
R-values:
R R work R free
0.172 0.171 0.19
Expression systems:
  • Drosophila
  • Not provided

Quick links

Citations

19 review citations

Hepatitis C Virus Infection: Host⁻Virus Interaction and Mechanisms of Viral Persistence.
Chigbu et al. (2019)
18 more

5 mentions without citation

Conformational Flexibility in the CD81-Binding Site of the Hepatitis C Virus Glycoprotein E2.
Ströh et al. (2018)
4 more