› 5fhc

X-ray diffraction
6.7Å resolution

Crystal Structure of Protective Human Antibodies 100 and 114 in Complex with Ebola Virus Fusion Glycoprotein (GP)

Released:
DOI: 10.2210/pdb5fhc/pdb
PDB entry 5fhc coloured by chain, front view.
PDB entry 5fhc coloured by chain, side view.
PDB entry 5fhc coloured by chain, top view.
Source organisms:
Primary publication:
Structural and molecular basis for Ebola virus neutralization by protective human antibodies.
Misasi J, Gilman MS, Kanekiyo M, Gui M, Cagigi A, Mulangu S, Corti D, Ledgerwood JE, Lanzavecchia A, Cunningham J, Muyembe-Tamfun JJ, Baxa U, Graham BS, Xiang Y, Sullivan NJ, McLellan JS
Science 351 1343-6 (2016)
PMID: 26917592

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211916 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
GP2 Chain: J
Molecule details ›
Chain: J
Length: 98 amino acids
Theoretical weight: 10.97 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 502-599; Coverage: 15%)
Gene name: GP
GP1 Chain: K
Molecule details ›
Chain: K
Length: 322 amino acids
Theoretical weight: 36.32 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 1-308, 490-501; Coverage: 45%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
Antibody 100 Fab heavy chain Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 24.23 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Antibody 100 Fab light chain Chain: B
Molecule details ›
Chain: B
Length: 207 amino acids
Theoretical weight: 22.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Antibody 114 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 22.94 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Antibody 114 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 169.67Å b: 169.67Å c: 376.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.26 0.343
Expression system: Homo sapiens

Quick links

Citations

28 review citations

Unravelling biological macromolecules with cryo-electron microscopy.
Fernandez-Leiro et al. (2016)
27 more

10 mentions without citation

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
9 more