› 5fhd

X-ray diffraction
2Å resolution

Structure of Bacteroides sp Pif1 complexed with tailed dsDNA resulting in ssDNA bound complex

Released:
DOI: 10.2210/pdb5fhd/pdb
PDB entry 5fhd coloured by chain, front view.
PDB entry 5fhd coloured by chain, side view.
PDB entry 5fhd coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Functional Insights into the Unwinding Mechanism of Bacteroides sp Pif1.
Zhou X, Ren W, Bharath SR, Tang X, He Y, Chen C, Liu Z, Li D, Song H
Cell Rep 14 2030-9 (2016)
PMID: 26904952

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-118675 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Uncharacterized protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 433 amino acids
Theoretical weight: 49.72 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*CP*CP*GP*GP*GP*GP*CP*CP*GP*CP*GP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 19 nucleotides
Theoretical weight: 5.79 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand ALF 2 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 48.471Å b: 149.64Å c: 185.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.212
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

The functions of the multi-tasking Pfh1Pif1 helicase.
Sabouri N. (2017)
5 more

7 mentions without citation

Structure and function of Pif1 helicase.
Byrd et al. (2017)
6 more