› 5fhe

X-ray diffraction
2.9Å resolution

Crystal structure of Bacteroides Pif1 bound to ssDNA

Released:
DOI: 10.2210/pdb5fhe/pdb
PDB entry 5fhe coloured by chain, front view.
PDB entry 5fhe coloured by chain, side view.
PDB entry 5fhe coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Functional Insights into the Unwinding Mechanism of Bacteroides sp Pif1.
Zhou X, Ren W, Bharath SR, Tang X, He Y, Chen C, Liu Z, Li D, Song H
Cell Rep 14 2030-9 (2016)
PMID: 26904952

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-118676 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Uncharacterized protein Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 49.72 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand ALF 1 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3121
Unit cell:
a: 87.442Å b: 87.442Å c: 149.822Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Structure and function of Pif1 helicase.
Byrd et al. (2017)
5 more

2 mentions without citation

Role and Regulation of Pif1 Family Helicases at the Replication Fork.
Malone et al. (2022)
1 more