› 5fhf

X-ray diffraction
2.15Å resolution

Crystal structure of Bacteroides sp Pif1 in complex with ADP-AlF4

Released:
DOI: 10.2210/pdb5fhf/pdb
PDB entry 5fhf coloured by chain, front view.
PDB entry 5fhf coloured by chain, side view.
PDB entry 5fhf coloured by chain, top view.
Source organism: Bacteroides fragilis
Primary publication:
Structural and Functional Insights into the Unwinding Mechanism of Bacteroides sp Pif1.
Zhou X, Ren W, Bharath SR, Tang X, He Y, Chen C, Liu Z, Li D, Song H
Cell Rep 14 2030-9 (2016)
PMID: 26904952

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 49.72 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand ALF 1 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 67.09Å b: 75.31Å c: 111.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.251
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and function of Pif1 helicase.
Byrd et al. (2017)
6 more

1 mention without citation

Structural Studies of Pif1 Helicases from Thermophilic Bacteria.
Réty et al. (2023)