› 5fjs

X-ray diffraction
2.6Å resolution

Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2)

Released:
DOI: 10.2210/pdb5fjs/pdb
PDB entry 5fjs coloured by chain, front view.
PDB entry 5fjs coloured by chain, side view.
PDB entry 5fjs coloured by chain, top view.
Source organism: Thermoanaerobacterium xylanolyticum LX-11
Primary publication:
Bacterial β-Glucosidase Reveals the Structural and Functional Basis of Genetic Defects in Human Glucocerebrosidase 2 (GBA2).
Charoenwattanasatien R, Pengthaisong S, Breen I, Mutoh R, Sansenya S, Hua Y, Tankrathok A, Wu L, Songsiriritthigul C, Tanaka H, Williams SJ, Davies GJ, Kurisu G, Cairns JR
ACS Chem Biol 11 1891-900 (2016)
PMID: 27115290

Function and Biology Details

Reaction catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosylceramidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 787 amino acids
Theoretical weight: 90.37 KDa
Source organism: Thermoanaerobacterium xylanolyticum LX-11
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F6BL85 (Residues: 21-806; Coverage: 98%)
Gene name: Thexy_2211
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P61
Unit cell:
a: 187.974Å b: 187.974Å c: 99.075Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

β-Xylosidases: Structural Diversity, Catalytic Mechanism, and Inhibition by Monosaccharides.
Rohman et al. (2019)
1 more

2 mentions without citation

Bacterial β-Glucosidase Reveals the Structural and Functional Basis of Genetic Defects in Human Glucocerebrosidase 2 (GBA2).
Charoenwattanasatien et al. (2016)
1 more