› 5fkf

X-ray diffraction
2.8Å resolution

SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is UC

Released:
DOI: 10.2210/pdb5fkf/pdb
PDB entry 5fkf coloured by chain, front view.
PDB entry 5fkf coloured by chain, side view.
PDB entry 5fkf coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
A critical base pair in k-turns determines the conformational class adopted, and correlates with biological function.
Huang L, Wang J, Lilley DM
Nucleic Acids Res 44 5390-8 (2016)
PMID: 27016741

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195850 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
SAM-I RIBOSWITCH Chain: A
Molecule details ›
Chain: A
Length: 94 nucleotides
Theoretical weight: 30.54 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
Sequence domains: SAM riboswitch (S box leader)

Ligands and Environments

4 bound ligands:
Ligand SAM 1 x SAM
Ligand BA 12 x BA
Ligand NA 2 x NA
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 61.81Å b: 61.81Å c: 154.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.258
Expression system: Not provided

Quick links

Citations

2 review citations

Adventures with RNA graphs.
Schlick T. (2018)
1 more

1 mention without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)