› 5fog

X-ray diffraction
2.3Å resolution

Crystal structure of hte Cryptosporidium muris cytosolic leucyl-tRNA synthetase editing domain complex with a post-transfer editing analogue of norvaline (Nv2AA)

Released:
DOI: 10.2210/pdb5fog/pdb
PDB entry 5fog coloured by chain, front view.
PDB entry 5fog coloured by chain, side view.
PDB entry 5fog coloured by chain, top view.
Source organism: Cryptosporidium muris RN66
Primary publication:
Cryptosporidium and Toxoplasma Parasites Are Inhibited by a Benzoxaborole Targeting Leucyl-tRNA Synthetase.
Palencia A, Liu RJ, Lukarska M, Gut J, Bougdour A, Touquet B, Wang ED, Li X, Alley MR, Freund YR, Rosenthal PJ, Hakimi MA, Cusack S
Antimicrob Agents Chemother 60 5817-27 (2016)
PMID: 27431220

Function and Biology Details

Reaction catalysed: 
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
leucine--tRNA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 32.97 KDa
Source organism: Cryptosporidium muris RN66
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: B6AA20 (Residues: 254-541; Coverage: 26%)
Gene name: CMU_041310
Sequence domains: tRNA synthetases class I (I, L, M and V)

Ligands and Environments

3 bound ligands:
Ligand VRT 4 x VRT
Ligand EDO 4 x EDO
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 107.73Å b: 107.73Å c: 309.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.236
Expression system: Escherichia coli K-12

Quick links

Citations

10 review citations

A Systematic Review of In vitro and In vivo Activities of Anti-Toxoplasma Drugs and Compounds (2006-2016).
Montazeri et al. (2017)
9 more

1 mention without citation

Cryptosporidium and Toxoplasma Parasites Are Inhibited by a Benzoxaborole Targeting Leucyl-tRNA Synthetase.
Palencia et al. (2016)