› 5fp2

X-ray diffraction
2.97Å resolution

Crystal structure of the siderophore receptor PirA from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5fp2/pdb
PDB entry 5fp2 coloured by chain, front view.
PDB entry 5fp2 coloured by chain, side view.
PDB entry 5fp2 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure and Function of the PiuA and PirA Siderophore-Drug Receptors from Pseudomonas aeruginosa and Acinetobacter baumannii.
Moynié L, Luscher A, Rolo D, Pletzer D, Tortajada A, Weingart H, Braun Y, Page MG, Naismith JH, Köhler T
Antimicrob Agents Chemother 61 (2017)
PMID: 28137795

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165661 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ferric enterobactin receptor PirA Chains: A, B
Molecule details ›
Chains: A, B
Length: 725 amino acids
Theoretical weight: 79.46 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I527 (Residues: 29-742; Coverage: 100%)
Gene names: PA0931, pirA
Sequence domains:
FERRIC ENTEROBACTIN RECEPTOR PIRA Chains: X, Z
Molecule details ›
Chains: X, Z
Length: 10 amino acids
Theoretical weight: 869 Da
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand OCT 3 x OCT
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 183.12Å b: 95.28Å c: 131.83Å
α: 90° β: 127.52° γ: 90°
R-values:
R R work R free
0.265 0.263 0.319
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Cefiderocol: Discovery, Chemistry, and In Vivo Profiles of a Novel Siderophore Cephalosporin.
Sato et al. (2019)
6 more

3 mentions without citation

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
2 more