› 5fqu

X-ray diffraction
2.74Å resolution

Orthorhombic crystal structure of of PlpD (selenomethionine derivative)

Released:
DOI: 10.2210/pdb5fqu/pdb
PDB entry 5fqu coloured by chain, front view.
PDB entry 5fqu coloured by chain, side view.
PDB entry 5fqu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural Basis of Lipid Targeting and Destruction by the Type V Secretion System of Pseudomonas aeruginosa.
da Mata Madeira PV, Zouhir S, Basso P, Neves D, Laubier A, Salacha R, Bleves S, Faudry E, Contreras-Martel C, Dessen A
J Mol Biol 428 1790-803 (2016)
PMID: 27012424

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PNPLA domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.29 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYQ6 (Residues: 20-333; Coverage: 44%)
Gene names: PA3339, plpD
Sequence domains: Patatin-like phospholipase

Ligands and Environments

2 bound ligands:
Ligand AZI 1 x AZI
Ligand NA 2 x NA
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 46.743Å b: 113.393Å c: 130.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.238
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.
Flores-Díaz et al. (2016)
6 more

2 mentions without citation

Deprivation of the Periplasmic Chaperone SurA Reduces Virulence and Restores Antibiotic Susceptibility of Multidrug-Resistant Pseudomonas aeruginosa.
Klein et al. (2019)
1 more