› 5fr6

X-ray diffraction
2.51Å resolution

The structure of polycomb ULD complex

Released:
DOI: 10.2210/pdb5fr6/pdb
PDB entry 5fr6 coloured by chain, front view.
PDB entry 5fr6 coloured by chain, side view.
PDB entry 5fr6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
BMI1 regulates PRC1 architecture and activity through homo- and hetero-oligomerization.
Gray F, Cho HJ, Shukla S, He S, Harris A, Boytsov B, Jaremko Ł, Jaremko M, Demeler B, Lawlor ER, Grembecka J, Cierpicki T
Nat Commun 7 13343 (2016)
PMID: 27827373

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polycomb complex protein BMI-1 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 14.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P35226 (Residues: 121-235; Coverage: 35%)
Gene names: BMI1, PCGF4, RNF51
Sequence domains: RAWUL domain RING finger- and WD40-associated ubiquitin-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3212
Unit cell:
a: 78.275Å b: 78.275Å c: 43.119Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.233 0.328
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Chromatin regulatory mechanisms and therapeutic opportunities in cancer.
Valencia et al. (2019)
9 more

2 mentions without citation

BMI1 regulates PRC1 architecture and activity through homo- and hetero-oligomerization.
Gray et al. (2016)
1 more