› 5ft4

X-ray diffraction
2Å resolution

Crystal structure of the cysteine desulfurase CsdA from Escherichia coli at 1.996 Angstroem resolution

Released:
DOI: 10.2210/pdb5ft4/pdb
PDB entry 5ft4 coloured by chain, front view.
PDB entry 5ft4 coloured by chain, side view.
PDB entry 5ft4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The Mechanism of Sulfur Transfer Across Protein- Protein Interfaces: The Csd Model
Fernandez FJ, Arda A, Lopez-Estepa M, Aranda J, Penya-Soler E, Garces F, Round A, Campos-Oliva R, Bruix M, Coll M, Tunon I, Jimenez-Barbero J, Vega MC
ACS Catalysis 6 3975- (2016)

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase CsdA Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46925 (Residues: 1-401; Coverage: 100%)
Gene names: JW2781, b2810, csdA, ygdJ
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand GOL 6 x GOL
Ligand CIT 4 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 64.095Å b: 102.561Å c: 71.258Å
α: 90° β: 114.45° γ: 90°
R-values:
R R work R free
0.14 0.138 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links