› 5ft8

X-ray diffraction
2.5Å resolution

Crystal structure of the complex between the cysteine desulfurase CsdA and the sulfur-acceptor CsdE in the persulfurated state at 2.50 Angstroem resolution

Released:
DOI: 10.2210/pdb5ft8/pdb
PDB entry 5ft8 coloured by chain, front view.
PDB entry 5ft8 coloured by chain, side view.
PDB entry 5ft8 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Mechanism of Sulfur Transfer Across Protein-Protein Interfaces: The Cysteine Desulfurase Model System
Fernandez FJ, Arda A, Lopez-Estepa M, Aranda J, Penya-Soler E, Garces F, Quintana JF, Round A, Campos-Oliva R, Bruix M, Coll M, Tunon I, Jimenez-Barbero J, Vega MC
ACS Catalysis 6 3975- (2016)

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142831 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine desulfurase CsdA Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 403 amino acids
Theoretical weight: 43.43 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46925 (Residues: 1-401; Coverage: 100%)
Gene names: JW2781, b2810, csdA, ygdJ
Sequence domains: Aminotransferase class-V
Structure domains:
Sulfur acceptor protein CsdE Chains: Q, S, U, W, Y, a, c, e
Molecule details ›
Chains: Q, S, U, W, Y, a, c, e
Length: 154 amino acids
Theoretical weight: 16.95 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGF2 (Residues: 1-147; Coverage: 100%)
Gene names: JW2782, b2811, csdE, ygdK
Sequence domains: Fe-S metabolism associated domain

Ligands and Environments


Cofactor: Ligand PLP 8 x PLP
2 bound ligands:
Ligand PEG 16 x PEG
Ligand GOL 51 x GOL
1 modified residue:
Ligand CSS 16 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 74.863Å b: 115.135Å c: 604.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.279
Expression system: Escherichia coli BL21(DE3)

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