› 5ftj

Electron Microscopy
2.3Å resolution

Cryo-EM structure of human p97 bound to UPCDC30245 inhibitor

Released:
DOI: 10.2210/pdb5ftj/pdb
PDB entry 5ftj coloured by chain, front view.
PDB entry 5ftj coloured by chain, side view.
PDB entry 5ftj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition.
Banerjee S, Bartesaghi A, Merk A, Rao P, Bulfer SL, Yan Y, Green N, Mroczkowski B, Neitz RJ, Wipf P, Falconieri V, Deshaies RJ, Milne JL, Huryn D, Arkin M, Subramaniam S
Science 351 871-5 (2016)
PMID: 26822609
Related structures: EMD-3295

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transitional endoplasmic reticulum ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 806 amino acids
Theoretical weight: 89.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55072 (Residues: 1-806; Coverage: 100%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 12 x ADP
Ligand OJA 6 x OJA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.3Å
Relevant EMDB volumes: EMD-3295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

50 review citations

Unravelling biological macromolecules with cryo-electron microscopy.
Fernandez-Leiro et al. (2016)
49 more

21 mentions without citation

Structure and function of the AAA+ ATPase p97/Cdc48p.
Xia et al. (2016)
20 more