› 5fuz

X-ray diffraction
2.68Å resolution

Extending the half-life of a Fab fragment through generation of a humanised anti-Human Serum Albumin (HSA) Fv domain: an investigation into the correlation between affinity and serum half-life

Released:
DOI: 10.2210/pdb5fuz/pdb
PDB entry 5fuz coloured by chain, front view.
PDB entry 5fuz coloured by chain, side view.
PDB entry 5fuz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Extending the half-life of a fab fragment through generation of a humanized anti-human serum albumin Fv domain: An investigation into the correlation between affinity and serum half-life.
Adams R, Griffin L, Compson JE, Jairaj M, Baker T, Ceska T, West S, Zaccheo O, Davé E, Lawson AD, Humphreys DP, Heywood S
MAbs 8 1336-1346 (2016)
PMID: 27315033

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213746 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
645 FAB, HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 233 amino acids
Theoretical weight: 24.9 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
645 FAB, LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3121
Unit cell:
a: 111.21Å b: 111.21Å c: 89.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.251
Expression system: Cricetulus griseus

Quick links

Citations

5 review citations

Antibody variable region engineering for improving cancer immunotherapy.
Lou et al. (2022)
4 more

2 mentions without citation

Extending the half-life of a fab fragment through generation of a humanized anti-human serum albumin Fv domain: An investigation into the correlation between affinity and serum half-life.
Adams et al. (2016)
1 more