› 5fvc

X-ray diffraction
4.17Å resolution

Structure of RNA-bound decameric HMPV nucleoprotein

Released:
DOI: 10.2210/pdb5fvc/pdb
Model geometry
Fit model/data
PDB entry 5fvc coloured by chain, front view.
PDB entry 5fvc coloured by chain, side view.
PDB entry 5fvc coloured by chain, top view.
Source organisms:
Primary publication:
Nucleocapsid assembly in pneumoviruses is regulated by conformational switching of the N protein.
Renner M, Bertinelli M, Leyrat C, Paesen GC, Saraiva de Oliveira LF, Huiskonen JT, Grimes JM
OpenAccess logo Elife 5 e12627 (2016)
PMID: 26880565

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187511 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 401 amino acids
Theoretical weight: 44.53 KDa
Source organism: Human metapneumovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q91F57 (Residues: 1-394; Coverage: 100%)
Gene name: N
Sequence domains: Pneumovirus nucleocapsid protein
RNA Chain: K
Molecule details ›
Chain: K
Length: 70 nucleotides
Theoretical weight: 21.32 KDa
Source organism: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 202.01Å b: 233.21Å c: 203.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

How order and disorder within paramyxoviral nucleoproteins and phosphoproteins orchestrate the molecular interplay of transcription and replication.
Longhi et al. (2017)
10 more

8 mentions without citation

Nucleocapsid Structure of Negative Strand RNA Virus.
Luo et al. (2020)
7 more