› 5fvn

X-ray diffraction
1.45Å resolution

X-ray crystal structure of Enterobacter cloacae OmpE36 porin.

Released:
DOI: 10.2210/pdb5fvn/pdb
PDB entry 5fvn coloured by chain, front view.
PDB entry 5fvn coloured by chain, side view.
PDB entry 5fvn coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis.
Arunmanee W, Pathania M, Solovyova AS, Le Brun AP, Ridley H, Baslé A, van den Berg B, Lakey JH
Proc Natl Acad Sci U S A 113 E5034-43 (2016)
PMID: 27493217

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Porin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 342 amino acids
Theoretical weight: 37.91 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q93K99 (Residues: 22-363; Coverage: 100%)
Gene names: EGK68_01615, NCTC10005_03257, ompC
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

Carbohydrate polymer : NEW Components: KDO, GP1, Z9M
Carbohydrate polymer
Carbohydrate polymer : NEW Components: KDO, GP1, Z9M, GMH
Carbohydrate polymer
Carbohydrate polymer : NEW Components: KDO, GP1, Z9M
Carbohydrate polymer
8 bound ligands:
Ligand SO4 2 x SO4
Ligand C8E 37 x C8E
Ligand PO4 6 x PO4
Ligand KDO 3 x KDO
Ligand FTT 36 x FTT
Ligand MYR 9 x MYR
Ligand DAO 15 x DAO
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 109.749Å b: 123.26Å c: 116.01Å
α: 90° β: 91.01° γ: 90°
R-values:
R R work R free
0.156 0.155 0.183
Expression system: Escherichia coli BL21

Quick links

Citations

16 review citations

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
15 more

7 mentions without citation

Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis.
Arunmanee et al. (2016)
6 more