› 5fyd

X-ray diffraction
1.6Å resolution

Structural and biochemical insights into 7beta-hydroxysteroid dehydrogenase stereoselectivity

Released:
DOI: 10.2210/pdb5fyd/pdb
PDB entry 5fyd coloured by chain, front view.
PDB entry 5fyd coloured by chain, side view.
PDB entry 5fyd coloured by chain, top view.
Source organism: Collinsella aerofaciens
Primary publication:
Structural and biochemical insights into 7β-hydroxysteroid dehydrogenase stereoselectivity.
Savino S, Ferrandi EE, Forneris F, Rovida S, Riva S, Monti D, Mattevi A
Proteins 84 859-65 (2016)
PMID: 27006087

Function and Biology Details

Reaction catalysed: 
A 7-beta-hydroxysteroid + NADP(+) = a 7-oxosteroid + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7beta-hydroxysteroid dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 28.78 KDa
Source organism: Collinsella aerofaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4ECA9 (Residues: 1-263; Coverage: 100%)
Gene name: COLAER_02088
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 114.77Å b: 83.04Å c: 47.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.209
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Biological synthesis of ursodeoxycholic acid.
Song et al. (2023)
4 other articles

2 mentions without citation

The effect of bisphenol A on testicular steroidogenesis and its amelioration by quercetin: an in vivo and in silico approach.
Samova et al. (2018)
1 more