› 5g08

X-ray diffraction
1.52Å resolution

Crystal structure of Drosophila NCS-1 bound to chlorpromazine

Released:
DOI: 10.2210/pdb5g08/pdb
Model geometry
Fit model/data
PDB entry 5g08 coloured by chain, front view.
PDB entry 5g08 coloured by chain, side view.
PDB entry 5g08 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Interference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome.
Mansilla A, Chaves-Sanjuan A, Campillo NE, Semelidou O, Martínez-González L, Infantes L, González-Rubio JM, Gil C, Conde S, Skoulakis EM, Ferrús A, Martínez A, Sánchez-Barrena MJ
Proc Natl Acad Sci U S A 114 E999-E1008 (2017)
PMID: 28119500

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194321 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Frequenin-2 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 21.92 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VWX8 (Residues: 1-187; Coverage: 100%)
Gene names: CG5907, Frq2
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

4 bound ligands:
Ligand CA 3 x CA
Ligand Z80 1 x Z80
Ligand GOL 2 x GOL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 54.217Å b: 54.917Å c: 60.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Inhibitors of protein-protein interactions (PPIs): an analysis of scaffold choices and buried surface area.
Ran et al. (2018)
7 more

2 mentions without citation

Interference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome.
Mansilla et al. (2017)
1 more