› 5g2f

X-ray diffraction
1.85Å resolution

Type IV-like competence pilin TTHA1222 from Thermus thermophilus

Released:
DOI: 10.2210/pdb5g2f/pdb
PDB entry 5g2f coloured by chain, front view.
PDB entry 5g2f coloured by chain, side view.
PDB entry 5g2f coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structures of type IV pilins from Thermus thermophilus demonstrate similarities with type II secretion system pseudopilins.
Karuppiah V, Thistlethwaite A, Derrick JP
J Struct Biol 196 375-384 (2016)
PMID: 27612581

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pilin A4 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 95 amino acids
Theoretical weight: 10.2 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIZ2 (Residues: 38-123; Coverage: 70%)
Gene name: TTHA1222
Sequence domains: Pilin A4
Structure domains: Pantoate--beta-alanine Ligase; Chain: A,domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I41
Unit cell:
a: 84.38Å b: 84.38Å c: 97.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.208
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of minor pilins of type IV pili.
Jacobsen et al. (2020)
1 more

1 mention without citation

Structures of type IV pilins from Thermus thermophilus demonstrate similarities with type II secretion system pseudopilins.
Karuppiah et al. (2016)