› 5g2q

X-ray diffraction
2.3Å resolution

The crystal structure of a S-selective transaminase from Arthrobacter sp. with alanine bound

Released:
DOI: 10.2210/pdb5g2q/pdb
PDB entry 5g2q coloured by chain, front view.
PDB entry 5g2q coloured by chain, side view.
PDB entry 5g2q coloured by chain, top view.
Source organism: Arthrobacter sp.
Primary publication:
Structural Basis of the Substrate Range and Enantioselectivity of Two (S)-Selective ω-Transaminases.
van Oosterwijk N, Willies S, Hekelaar J, Terwisscha van Scheltinga AC, Turner NJ, Dijkstra BW
Biochemistry 55 4422-31 (2016)
PMID: 27428867

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transaminase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 485 amino acids
Theoretical weight: 54.2 KDa
Source organism: Arthrobacter sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1C7D191 (Residues: 1-485; Coverage: 100%)
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PDA 12 x PDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 64.278Å b: 99.814Å c: 217.337Å
α: 81.58° β: 89.12° γ: 74.49°
R-values:
R R work R free
0.192 0.192 0.249
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

High-Yield Synthesis of Enantiopure 1,2-Amino Alcohols from l-Phenylalanine via Linear and Divergent Enzymatic Cascades.
Corrado et al. (2022)
1 more