› 5g3p

X-ray diffraction
1.78Å resolution

Bacillus cereus formamidase (BceAmiF) acetylated at the active site.

Released:
DOI: 10.2210/pdb5g3p/pdb
PDB entry 5g3p coloured by chain, front view.
PDB entry 5g3p coloured by chain, side view.
PDB entry 5g3p coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
A novel cysteine carbamoyl-switch is responsible for the inhibition of formamidase, a nitrilase superfamily member.
Martínez-Rodríguez S, Conejero-Muriel M, Gavira JA
Arch Biochem Biophys 662 151-159 (2019)
PMID: 30528776

Function and Biology Details

Reaction catalysed: 
Formamide + H(2)O = formate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formamidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 346 amino acids
Theoretical weight: 38.72 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P59701 (Residues: 1-332; Coverage: 100%)
Gene names: BC_3939, amiF
Sequence domains: Carbon-nitrogen hydrolase
Structure domains: Carbon-nitrogen hydrolase

Ligands and Environments

7 bound ligands:
Ligand PEG 12 x PEG
Ligand EDO 3 x EDO
Ligand ACT 4 x ACT
Ligand NA 15 x NA
Ligand PGE 6 x PGE
Ligand PG4 1 x PG4
Ligand PO4 2 x PO4
1 modified residue:
Ligand SCY 6 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2
Unit cell:
a: 143.764Å b: 150.208Å c: 98.257Å
α: 90° β: 98.68° γ: 90°
R-values:
R R work R free
0.164 0.164 0.181
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Prospects of formamide as nitrogen source in biotechnological production processes.
Schwardmann et al. (2024)